Hotspot

rps8-rpl14

Genome · 83209-83931 · 723 bp

Back to Wisteria

Pi 0.0199

MarkerSeek score 72.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 723
variable_sites: 14
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCATTGCGTATCAAGGT	TGTTTCATTAGCACCCGAGG	45.0	50.0	57.6	57.9	801-835 bp	1.0	79.2
rps8-rpl14_p2	TCAGCATTGCGTATCAAGGT	GTTTCATTAGCACCCGAGGT	45.0	50.0	57.6	57.9	800-834 bp	1.0	79.2
rps8-rpl14_p3	GTCAGCATTGCGTATCAAGG	TGTTTCATTAGCACCCGAGG	50.0	50.0	57.3	57.9	802-836 bp	1.0	76.5
rps8-rpl14_p4	GTCAGCATTGCGTATCAAGG	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.3	57.9	801-835 bp	1.0	76.5
rps8-rpl14_p5	TCAGCATTGCGTATCAAGGT	CCGGGAATTGAGACAGTTGA	45.0	50.0	57.6	57.5	834-868 bp	1.0	76.2