Hotspot

trnP-UGG-psaJ

Genome · 68923-69375 · 453 bp

Back to Wisteria

Pi 0.0181

MarkerSeek score 65.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 453
variable_sites: 8
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGCTAAAACCCCGAACCATA	50.0	50.0	58.1	58.0	577-583 bp	1.0	81.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGCTAAAACCCCGAACCATA	50.0	50.0	58.1	58.0	576-582 bp	1.0	81.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGCTAAAACCCCGAACCATA	50.0	50.0	58.1	58.0	588-594 bp	1.0	81.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGCTAAAACCCCGAACCATA	50.0	50.0	58.1	58.0	587-593 bp	1.0	81.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGCTAAAACCCCGAACCATA	50.0	50.0	58.1	58.0	590-596 bp	1.0	81.6