Hotspot

psbD-trnT-GGU

Genome · 29967-31487 · 1521 bp

Back to Wisteria

Pi 0.011

MarkerSeek score 64.40

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1521
variable_sites: 16
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CGAATCGATCCCTCCGTAAC	CAGTGTGGTAGAGTAACGCC	55.0	55.0	58.0	58.0	1609-1668 bp	1.0	83.6
psbD-trnT-GGU_p2	AAAACGAATCGATCCCTCCG	CAGTGTGGTAGAGTAACGCC	50.0	55.0	58.1	58.0	1613-1672 bp	1.0	83.5
psbD-trnT-GGU_p3	CGAATCGATCCCTCCGTAAC	ACTCCTATTGGCTGGTTCCT	55.0	50.0	58.0	58.0	1665-1724 bp	1.0	83.3
psbD-trnT-GGU_p4	AAAACGAATCGATCCCTCCG	ACTCCTATTGGCTGGTTCCT	50.0	50.0	58.1	58.0	1669-1728 bp	1.0	83.2
psbD-trnT-GGU_p5	CGAATCGATCCCTCCGTAAC	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.0	58.1	1605-1664 bp	1.0	83.2