Hotspot

petG-trnW-CCA

LSC · 69474-69631 · 158 bp

Back to Wikstroemia

Pi 0.0352

MarkerSeek score 71.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 18
pi: 0.0352
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CAATACAGACGTGGCGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	254-268 bp	0.9	85.1
petG-trnW-CCA_p2	GGCTGGACTATTCGTGACTG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.1	58.0	284-296 bp	0.9	83.6
petG-trnW-CCA_p3	TTCATTTAGCCCCCTCATGC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	642-655 bp	0.9	80.8
petG-trnW-CCA_p4	ACAATACAGACGTGGCGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	257-269 bp	0.7	75.2
petG-trnW-CCA_p5	CCTGCCATGCCAGTCTATTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	817-832 bp	0.7	72.8