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psbZ-trnG-GCC

LSC · 38443-39102 · 660 bp

Back to Wikstroemia

Pi 0.0361

MarkerSeek score 76.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 77
pi: 0.0361
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAACCACTCAGCCATCTCTC	CGCCGATCCTCAAAGAAAGA	55.0	50.0	58.0	58.0	1791-1956 bp	1.0	88.8
psbZ-trnG-GCC_p2	GAGCTATCAACCACTCAGCC	CGCCGATCCTCAAAGAAAGA	55.0	50.0	58.1	58.0	1798-1963 bp	1.0	88.5
psbZ-trnG-GCC_p3	GGAGCTATCAACCACTCAGC	CGCCGATCCTCAAAGAAAGA	55.0	50.0	58.1	58.0	1799-1964 bp	1.0	88.5
psbZ-trnG-GCC_p4	CAAGACCGGAGCTATCAACC	CGCCGATCCTCAAAGAAAGA	55.0	50.0	58.1	58.0	1806-1971 bp	1.0	88.5
psbZ-trnG-GCC_p5	TAGCTTTCCAATTGGCCGTT	CGCCGATCCTCAAAGAAAGA	45.0	50.0	58.1	58.0	1458-1565 bp	0.9	84.4