Hotspot

trnT-GGU-psbD

LSC · 33828-35094 · 1267 bp

Back to Wikstroemia

Pi 0.0186

MarkerSeek score 71.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 83
pi: 0.0186
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCCTACGTAACCAGTCATCC	55.0	55.0	58.1	57.1	1381-1430 bp	1.0	80.8
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	CCCTACGTAACCAGTCATCC	50.0	55.0	58.3	57.1	1358-1407 bp	1.0	79.2
trnT-GGU-psbD_p3	GGCCCTTTTAACTCAGTGGT	CCCTACGTAACCAGTCATCC	50.0	55.0	57.7	57.1	1396-1445 bp	1.0	79.0
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	CCCTACGTAACCAGTCATCC	50.0	55.0	58.4	57.1	1380-1429 bp	1.0	77.9
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CCCTACGTAACCAGTCATCC	50.0	55.0	58.1	57.1	1389-1433 bp	0.9	75.6