Hotspot

psbM-trnD-GUC

LSC · 31680-32454 · 775 bp

Back to Wikstroemia

Pi 0.0179

MarkerSeek score 72.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 52
pi: 0.0179
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATCAACAGCGCAGTAGCAAT	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.3	57.8	783-885 bp	1.0	84.8
psbM-trnD-GUC_p2	AATTGCCTTGGCTGACTGTT	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.3	57.8	842-944 bp	1.0	84.3
psbM-trnD-GUC_p3	ATTGCCTTGGCTGACTGTTT	AGTTCAACTGGTCAGAGCAC	45.0	50.0	58.3	57.8	841-943 bp	1.0	84.3
psbM-trnD-GUC_p4	TCAACAGCGCAGTAGCAATA	AGTTCAACTGGTCAGAGCAC	45.0	50.0	57.6	57.8	782-884 bp	1.0	83.9
psbM-trnD-GUC_p5	ATCAACAGCGCAGTAGCAAT	ACGTGTATGCTGCACACTTT	45.0	45.0	58.3	58.4	822-924 bp	1.0	83.8