Hotspot

petN-psbM

LSC · 30448-31574 · 1127 bp

Back to Wikstroemia

Pi 0.0242

MarkerSeek score 71.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 95
pi: 0.0242
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ACTCGTACCCATGGATGACA	45.0	50.0	58.0	58.1	1826-1960 bp	1.0	88.7
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	GGTTGTACAAGGAAAGGGGG	45.0	55.0	58.0	58.1	1599-1682 bp	0.9	84.5
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	GGAAAGGGGGCAATTGGTTA	45.0	50.0	58.0	58.1	1589-1672 bp	0.8	80.1
petN-psbM_p4	AGGGGGCATCTTTCTTTGTG	GGAAAGGGGGCAATTGGTTA	50.0	50.0	58.1	58.1	2049-2133 bp	0.8	79.0
petN-psbM_p5	CAGGGGGCATCTTTCTTTGT	GGAAAGGGGGCAATTGGTTA	50.0	50.0	58.1	58.1	2050-2134 bp	0.8	79.0