Hotspot

trnS-GCU-trnG-UCC

LSC · 8837-9607 · 771 bp

Back to Wikstroemia

Pi 0.0366

MarkerSeek score 77.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 83
pi: 0.0366
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	ACGGGGTTAATAGAACGAATCA	57.9	40.9	58.1	57.3	631-838 bp	1.0	61.3
trnS-GCU-trnG-UCC_p2	TCCACTCAGCCATCTCTCC	CGGGGTTAATAGAACGAATCAC	57.9	45.5	58.1	57.0	630-837 bp	1.0	58.9
trnS-GCU-trnG-UCC_p3	GTCCACTCAGCCATCTCTCC	ACGGGGTTAATAGAACGAATCA	60.0	40.9	59.5	57.3	632-839 bp	1.0	57.8
trnS-GCU-trnG-UCC_p4	GTCCACTCAGCCATCTCTCC	CGGGGTTAATAGAACGAATCAC	60.0	45.5	59.5	57.0	631-838 bp	1.0	55.3
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	CGGGGTTAATAGAACGAATCA	57.9	42.9	58.1	55.5	630-837 bp	1.0	55.3