Hotspot

trnH-GUG-psbA

LSC · 96-425 · 330 bp

Back to Wikstroemia

Pi 0.0427

MarkerSeek score 74.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 330
variable_sites: 46
pi: 0.0427
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TGAAGCTCCATCTACAAATGG	50.0	42.9	57.9	55.7	374-399 bp	1.0	64.2
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TGAAGCTCCATCTACAAATGG	50.0	42.9	58.2	55.7	375-400 bp	1.0	64.0
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TGAAGCTCCATCTACAAATGG	50.0	42.9	57.7	55.7	367-392 bp	1.0	62.6
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAAGCTCCATCTACAAATGG	50.0	40.9	57.9	56.4	375-400 bp	1.0	62.2
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TTGAAGCTCCATCTACAAATGG	50.0	40.9	58.2	56.4	376-401 bp	1.0	62.0