Hotspot

rps4

Genome · 7549-8154 · 606 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 606
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TCCTCTTTCCAACATTATTTGTTCT	ACAGAGGAACAGATGTGCAT	32.0	45.0	56.9	56.8	698 bp	1.0	40.0
rps4_p2	TCCTCTTTCCAACATTATTTGTTC	ACAGAGGAACAGATGTGCAT	33.3	45.0	55.6	56.8	698 bp	1.0	40.0
rps4_p3	TCCTCTTTCCAACATTATTTGT	ACAGAGGAACAGATGTGCAT	31.8	45.0	53.6	56.8	698 bp	1.0	40.0
rps4_p4	CCTCTTTCCAACATTATTTGTTCT	ACAGAGGAACAGATGTGCAT	33.3	45.0	55.6	56.8	697 bp	1.0	40.0
rps4_p5	TCCTCTTTCCAACATTATTTGTTCTT	ACAGAGGAACAGATGTGCAT	30.8	45.0	57.5	56.8	698 bp	1.0	40.0