Hotspot

psbD

Genome · 18789-19850 · 1062 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	GCCACATGAGCTCCAAGTAA	TGAAAGGGATGATGGATGAA	50.0	40.0	57.9	54.0	1285 bp	1.0	46.8
psbD_p2	GCCACATGAGCTCCAAGTAA	TTGAAAGGGATGATGGATGA	50.0	40.0	57.9	54.0	1286 bp	1.0	46.8
psbD_p3	CACCAAGCGAAACCAGTAGT	TGAAAGGGATGATGGATGAA	50.0	40.0	58.1	54.0	1228 bp	1.0	46.8
psbD_p4	CACCAAGCGAAACCAGTAGT	TTGAAAGGGATGATGGATGA	50.0	40.0	58.1	54.0	1229 bp	1.0	46.8
psbD_p5	GCCACATGAGCTCCAAGTAA	TGAAAGGGATGATGGATGAAA	50.0	38.1	57.9	54.9	1285 bp	1.0	45.9