Hotspot

psbC

Genome · 17420-18841 · 1422 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	CCGAGCACCAAAGATTCAGT	GAGATTCGTGCAGCAGAAGA	50.0	50.0	58.2	58.0	1595 bp	1.0	78.5
psbC_p2	CCGAGCACCAAAGATTCAGT	GTGCAGCAGAAGATCCTGAA	50.0	50.0	58.2	57.9	1588 bp	1.0	77.7
psbC_p3	CCGAGCACCAAAGATTCAGT	AGGTATTCGTGCTTGGATGG	50.0	50.0	58.2	57.7	1530 bp	1.0	75.9
psbC_p4	CCGAGCACCAAAGATTCAGT	ATTCGTGCAGCAGAAGATCC	50.0	50.0	58.2	58.3	1592 bp	1.0	75.8
psbC_p5	CCGAGCACCAAAGATTCAGT	TGTTTCCCAGGAGATTCGTG	50.0	50.0	58.2	57.5	1605 bp	1.0	74.7