Hotspot

matK

Genome · 1722-3242 · 1521 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: Genome
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGTAGGGATTGATCCTGGCA	ATAATCCAAGAGGGGTCCGG	50.0	55.0	58.1	58.6	1598 bp	1.0	74.6
matK_p2	TTTCATTGCACACGGCTTTC	ATAATCCAAGAGGGGTCCGG	45.0	55.0	57.9	58.6	1672 bp	1.0	74.4
matK_p3	GCACACGGCTTTCTCCATAT	ATAATCCAAGAGGGGTCCGG	50.0	55.0	58.3	58.6	1665 bp	1.0	72.8
matK_p4	CTGTAGGGATTGATCCTGGC	ATAATCCAAGAGGGGTCCGG	55.0	55.0	57.4	58.6	1599 bp	1.0	71.0
matK_p5	TTTTCATTGCACACGGCTTT	ATAATCCAAGAGGGGTCCGG	40.0	55.0	57.4	58.6	1673 bp	1.0	70.7