Hotspot

accD-ycf1

Genome · 103159-104249 · 1091 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 63.40

Primer available yes

Label accD-ycf1

Feature Metadata

label_name: accD-ycf1
feature_type: igs
region: Genome
length_bp: 1091
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-ycf1_p1	CATGGTTCCACGAGCTCTTT	ATATATTTGCGCCGGGCC	50.0	55.6	58.2	58.0	1226-1287 bp	1.0	66.4
accD-ycf1_p2	CATGGTTCCACGAGCTCTTT	TCCTTCTATCCAGCGAATGA	50.0	45.0	58.2	55.7	1151-1212 bp	1.0	64.4
accD-ycf1_p3	ATGGTTCCACGAGCTCTTTT	ATATATTTGCGCCGGGCC	45.0	55.6	57.4	58.0	1225-1286 bp	1.0	63.3
accD-ycf1_p4	TGATAGCATGGTTCCACGAG	ATATATTTGCGCCGGGCC	50.0	55.6	57.4	58.0	1232-1293 bp	1.0	63.0
accD-ycf1_p5	CATGGTTCCACGAGCTCTTT	GATCCTTCTATCCAGCGAATGA	50.0	45.5	58.2	57.5	1153-1214 bp	1.0	62.3