Hotspot

trnK-UUU-chlB

Genome · 3922-5313 · 1392 bp

Back to Widdringtonia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-chlB

Feature Metadata

label_name
trnK-UUU-chlB
feature_type
igs
region
Genome
length_bp
1392
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-chlB_p1 AGTCGCACTTAAAAGCCGAG TTGCACGACAAAAGGGGATT 50.0 45.0 58.6 58.3 1511 bp 1.0 73.0
trnK-UUU-chlB_p2 AGTCGCACTTAAAAGCCGAG TTTGCACGACAAAAGGGGAT 50.0 45.0 58.6 58.3 1512 bp 1.0 73.0
trnK-UUU-chlB_p3 TAGTCGCACTTAAAAGCCGA TTGCACGACAAAAGGGGATT 45.0 45.0 57.3 58.3 1512 bp 1.0 71.8
trnK-UUU-chlB_p4 TCGCACTTAAAAGCCGAGTA TTGCACGACAAAAGGGGATT 45.0 45.0 57.3 58.3 1509 bp 1.0 71.8
trnK-UUU-chlB_p5 TCGCACTTAAAAGCCGAGTA TTTGCACGACAAAAGGGGAT 45.0 45.0 57.3 58.3 1510 bp 1.0 71.8