Hotspot

rps8

LSC · 126834-127238 · 405 bp

Back to Werauhia

Pi 0

MarkerSeek score 21.10

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TATAAAACTGCGGCGGATCC	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.1	58.0	895-897 bp	1.0	79.3
rps8_p2	TATAATTCGTCCACGCCTGC	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.1	58.0	1177-1179 bp	1.0	79.2
rps8_p3	ACCGTTGGGAAGTGATTCAG	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.8	58.0	820-822 bp	1.0	78.9
rps8_p4	CCGTTGGGAAGTGATTCAGT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.8	58.0	819-821 bp	1.0	78.9
rps8_p5	TATAAAACTGCGGCGGATCC	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	58.1	58.1	893-895 bp	1.0	78.5