Hotspot

accD

LSC · 103198-104664 · 1467 bp

Back to Werauhia

Pi 0.00477

MarkerSeek score 61.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 7
pi: 0.00477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAAATAGGGGGCAGGAAGA	ACTTGTTCGCTACAAACAAA	50.0	35.0	58.0	54.0	1534 bp	1.0	48.1
accD_p2	TCATCAAATAGGGGGCAGGA	ACTTGTTCGCTACAAACAAA	50.0	35.0	58.1	54.0	1537 bp	1.0	47.5
accD_p3	TCAAATAGGGGGCAGGAAGA	ACGATGAACTAAATACTTGTTCGC	50.0	37.5	58.0	57.9	1548 bp	1.0	47.5
accD_p4	TCAAATAGGGGGCAGGAAGA	ACTTGTTCGCTACAAACAAAA	50.0	33.3	58.0	54.8	1534 bp	1.0	47.0
accD_p5	TCATCAAATAGGGGGCAGGA	ACGATGAACTAAATACTTGTTCGC	50.0	37.5	58.1	57.9	1551 bp	1.0	46.9