Hotspot

rps3-rpl22

LSC · 130180-130225 · 46 bp

Back to Werauhia

Pi 0.0217

MarkerSeek score 64.60

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 46
variable_sites: 1
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGTAGGCCCCCGGAATAATT	GAAAAAGTTAAGACCGCGGG	50.0	50.0	58.5	57.4	265 bp	1.0	71.6
rps3-rpl22_p2	AGGCCCCCGGAATAATTTTT	GAAAAAGTTAAGACCGCGGG	45.0	50.0	57.4	57.4	262 bp	1.0	71.0
rps3-rpl22_p3	GTTGTGCGAACCAAAAGGAA	GAAAAAGTTAAGACCGCGGG	45.0	50.0	57.4	57.4	240 bp	1.0	70.9
rps3-rpl22_p4	GTAGGCCCCCGGAATAATTT	GAAAAAGTTAAGACCGCGGG	50.0	50.0	57.3	57.4	264 bp	1.0	70.1
rps3-rpl22_p5	TTCATCTTCCTGTAGGCCCC	GAAAAAGTTAAGACCGCGGG	55.0	50.0	58.8	57.4	275 bp	1.0	69.1