Hotspot

rps8-rpl14

LSC · 127239-127394 · 156 bp

Back to Werauhia

Pi 0.0321

MarkerSeek score 70.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 5
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATCGGCAATAGTGTCCCT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.2	58.0	268-270 bp	1.0	79.7
rps8-rpl14_p2	AGATCGGCAATAGTGTCCCT	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	58.2	58.1	266-268 bp	1.0	78.9
rps8-rpl14_p3	AGATCGGCAATAGTGTCCCT	AACTCGAGTTTTTGGTGCGA	50.0	45.0	58.2	58.3	267-269 bp	1.0	77.1
rps8-rpl14_p4	AGATCGGCAATAGTGTCCCT	GGAACTCGAGTTTTTGGTGC	50.0	50.0	58.2	57.6	269-271 bp	1.0	76.6
rps8-rpl14_p5	AGATCGGCAATAGTGTCCCT	CTCGAGTTTTTGGTGCGATC	50.0	50.0	58.2	57.5	265-267 bp	1.0	75.8