Hotspot

rpl36-infA

LSC · 126376-126483 · 108 bp

Back to Werauhia

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 2
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TATAATTCGTCCACGCCTGC	TGATTCAACCAGGGGACGTA	50.0	50.0	58.1	58.4	238 bp	1.0	76.7
rpl36-infA_p2	TTTTACGAACGGAAGCCCTT	TGATTCAACCAGGGGACGTA	45.0	50.0	57.7	58.4	194 bp	1.0	75.9
rpl36-infA_p3	TATAATTCGTCCACGCCTGC	CGCAACAAAGATTCGAACGA	50.0	45.0	58.1	57.4	204 bp	1.0	75.1
rpl36-infA_p4	TATAATTCGTCCACGCCTGC	TCGCAACAAAGATTCGAACG	50.0	45.0	58.1	57.4	205 bp	1.0	75.1
rpl36-infA_p5	TTTTACGAACGGAAGCCCTT	CGCAACAAAGATTCGAACGA	45.0	45.0	57.7	57.4	160 bp	1.0	74.4