Hotspot

petA-psbJ

LSC · 109004-110139 · 1136 bp

Back to Werauhia

Pi 0.00443
MarkerSeek score 62.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1136
variable_sites
5
pi
0.00443
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCTGGGTTGGGTTCATCTCT 50.0 50.0 57.9 58.0 1254-1262 bp 1.0 80.1
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC TGGGTTGGGTTCATCTCTCT 50.0 50.0 57.9 58.0 1252-1260 bp 1.0 80.1
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CTGGGTTGGGTTCATCTCTC 50.0 55.0 57.9 57.6 1253-1261 bp 1.0 77.1
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.7 58.0 1261-1269 bp 1.0 75.0
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTCT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.7 58.0 1259-1267 bp 1.0 75.0