Hotspot

trnF-GAA-ndhJ

LSC · 94012-94777 · 766 bp

Back to Werauhia

Pi 0.00399

MarkerSeek score 62.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 3
pi: 0.00399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	856-866 bp	1.0	79.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGCTG	50.0	55.0	58.1	58.3	868-878 bp	1.0	78.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGCTGG	50.0	55.0	58.1	58.3	867-877 bp	1.0	78.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	871-881 bp	1.0	78.4
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.2	58.2	898-908 bp	1.0	78.3