Hotspot

rps18

LSC · 71729-72202 · 474 bp

Back to Weigela

Pi 0.00914

MarkerSeek score 66.50

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 474
variable_sites: 6
pi: 0.00914
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CGACCGAAATAGGATTTTTGGG	CGAGCAAAACCTCAATCTGC	45.5	50.0	57.7	57.7	565-628 bp	1.0	63.3
rps18_p2	CGACCGAAATAGGATTTTTGGG	GAGCAAAACCTCAATCTGCG	45.5	50.0	57.7	57.7	564-627 bp	1.0	63.3
rps18_p3	CGACCGAAATAGGATTTTTGGG	AGCAAAACCTCAATCTGCGT	45.5	45.0	57.7	58.4	563-626 bp	1.0	62.2
rps18_p4	CGACCGAAATAGGATTTTTGGG	GCAAAACCTCAATCTGCGTT	45.5	45.0	57.7	57.6	562-625 bp	1.0	62.0
rps18_p5	CGACCGAAATAGGATTTTTGGG	ACCTCAATCTGCGTTTGAGT	45.5	45.0	57.7	57.4	557-620 bp	1.0	61.0