Hotspot

rpoA

LSC · 81885-82880 · 996 bp

Back to Weigela

Pi 0.0141

MarkerSeek score 53.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 21
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AAACCCATTTTCCGCTTCCT	CAGATGTACGGGGTCAAGTG	45.0	55.0	58.0	58.0	1412-1430 bp	1.0	81.1
rpoA_p2	AACCCATTTTCCGCTTCCTT	CAGATGTACGGGGTCAAGTG	45.0	55.0	58.0	58.0	1411-1429 bp	1.0	81.1
rpoA_p3	ACCCATTTTCCGCTTCCTTT	CAGATGTACGGGGTCAAGTG	45.0	55.0	58.0	58.0	1410-1428 bp	1.0	81.1
rpoA_p4	CCGCGGGATTGTTAACAGTA	CAGATGTACGGGGTCAAGTG	50.0	55.0	58.0	58.0	1732-1750 bp	1.0	80.8
rpoA_p5	TAATGGTTTCAGTACCCGCG	CAGATGTACGGGGTCAAGTG	50.0	55.0	58.0	58.0	1747-1765 bp	1.0	80.8