Hotspot

ycf1-trnN-GUU

IRa · 133503-134042 · 540 bp

Back to Weigela

Pi 0.102

MarkerSeek score 73.30

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 540
variable_sites: 52
pi: 0.102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GGGCCCTATGAAGAATGTGG	GGCAGAATAGCAGAGCAAGT	55.0	50.0	58.0	58.0	905-1103 bp	1.0	89.2
ycf1-trnN-GUU_p2	GGGCCCTATGAAGAATGTGG	GAACTTGGGTGTGGGTCTTT	55.0	50.0	58.0	57.9	1019-1217 bp	1.0	88.5
ycf1-trnN-GUU_p3	GGGCCCTATGAAGAATGTGG	GGGTGTGGGTCTTTTGTCTT	55.0	50.0	58.0	57.9	1013-1211 bp	1.0	88.5
ycf1-trnN-GUU_p4	GGGCCCTATGAAGAATGTGG	CACAACCTTCCCAAAAGGGA	55.0	50.0	58.0	57.9	1098-1296 bp	1.0	88.1
ycf1-trnN-GUU_p5	GGGCCCTATGAAGAATGTGG	ATCATGATCGGGATAGCGGA	55.0	50.0	58.0	58.1	1051-1249 bp	1.0	87.8