Hotspot

trnN-GUU-ndhF

IRb · 113220-115386 · 2167 bp

Back to Weigela

Pi 0.0476

MarkerSeek score 86.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2167
variable_sites: 142
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	CTGGAAGTGGAAGGAAAGGT	50.0	50.0	58.2	57.0	2136-2565 bp	1.0	80.0
trnN-GUU-ndhF_p2	GGATTCGAACCTACGACCAG	CTGGAAGTGGAAGGAAAGGT	55.0	50.0	57.8	57.0	2135-2564 bp	1.0	79.6
trnN-GUU-ndhF_p3	CCCCAAGTAGGATTCGAACC	CTGGAAGTGGAAGGAAAGGT	55.0	50.0	57.7	57.0	2144-2573 bp	1.0	78.6
trnN-GUU-ndhF_p4	TCTCCCCAAGTAGGATTCGA	CTGGAAGTGGAAGGAAAGGT	50.0	50.0	57.2	57.0	2147-2576 bp	1.0	74.5
trnN-GUU-ndhF_p5	AGGATTCGAACCTACGACCA	TGTTGCTGTCGGAAAAAGAAG	50.0	42.9	58.2	57.3	2183-2612 bp	1.0	74.0