Hotspot

trnI-CAU-ycf2

IRb · 90419-90695 · 277 bp

Back to Weigela

Pi 0.0169

MarkerSeek score 63.60

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 277
variable_sites: 7
pi: 0.0169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	AGGGTTGTAGTCTTCTCATTGTG	50.0	43.5	58.0	58.1	331-332 bp	1.0	57.1
trnI-CAU-ycf2_p2	ATTGGCATGCATCCAGTAGG	AGGGTTGTAGTCTTCTCATTGTG	50.0	43.5	58.0	58.1	380-381 bp	1.0	56.8
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	CTGAATTGAATTCGGTCCATGA	50.0	40.9	58.0	56.8	412-413 bp	1.0	55.6
trnI-CAU-ycf2_p4	ATTGGCATGCATCCAGTAGG	AGGGTTGTAGTCTTCTCATTGT	50.0	40.9	58.0	56.7	380-381 bp	1.0	55.6
trnI-CAU-ycf2_p5	ATTGGCATGCATCCAGTAGG	CTGAATTGAATTCGGTCCATGA	50.0	40.9	58.0	56.8	461-462 bp	1.0	55.5