Hotspot

petA-psbJ

LSC · 66334-67387 · 1054 bp

Back to Weigela

Pi 0.0016

MarkerSeek score 61.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 2
pi: 0.0016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1120-1165 bp	1.0	80.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1154-1199 bp	1.0	80.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1137-1182 bp	1.0	79.2
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1151-1196 bp	1.0	79.0
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1149-1194 bp	1.0	77.6