Hotspot

ycf4-cemA

LSC · 63636-64450 · 815 bp

Back to Weigela

Pi 0.0033

MarkerSeek score 61.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 4
pi: 0.0033
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	919-927 bp	1.0	79.7
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	935-943 bp	1.0	79.2
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CAGGGCAAAAAGACGAGAGA	45.0	50.0	58.1	57.8	908-916 bp	1.0	78.9
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	CAGGGCAAAAAGACGAGAGA	45.0	50.0	57.8	57.8	924-932 bp	1.0	78.5
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	ATCCACCAGGGCAAAAAGAC	45.0	50.0	58.1	58.4	914-922 bp	1.0	77.3