Hotspot

trnT-GGU-psbD

LSC · 34263-35111 · 849 bp

Back to Weigela

Pi 0.00393

MarkerSeek score 61.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 5
pi: 0.00393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CAAAGAGGCCCCTTTAGCTC	GGACCAGCCTACAAAAACGA	55.0	50.0	58.2	58.1	1036-1039 bp	1.0	77.5
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	992-995 bp	1.0	77.2
trnT-GGU-psbD_p3	CAAAGAGGCCCCTTTAGCTC	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.2	57.8	1009-1012 bp	1.0	76.6
trnT-GGU-psbD_p4	AATCCGATAAGGGGCTTTGG	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	973-976 bp	1.0	76.6
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.3	57.8	965-968 bp	1.0	76.3