Hotspot

trnH-GUG-psbA

LSC · 69-412 · 344 bp

Back to Weigela

Pi 0.0489
MarkerSeek score 71.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
344
variable_sites
25
pi
0.0489
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA GAGCTTAGTTTCCGTCTGGG 50.0 55.0 57.9 58.0 1046-1049 bp 1.0 81.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GGAGCTTAGTTTCCGTCTGG 50.0 55.0 57.9 58.0 1047-1050 bp 1.0 81.1
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GGGAGCTTAGTTTCCGTCTG 50.0 55.0 57.9 58.0 1048-1051 bp 1.0 81.1
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1052-1055 bp 1.0 81.0
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GAGCTTAGTTTCCGTCTGGG 50.0 55.0 57.9 58.0 1047-1050 bp 1.0 81.0