Hotspot

rpl2

IRa · 155798-157281 · 1484 bp

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Pi 0

MarkerSeek score 38.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1484
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.3	58.1	1671 bp	1.0	76.8
rpl2_p2	CGCTTCAACCGGGTTATTCT	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.3	57.9	1657 bp	1.0	76.8
rpl2_p3	CGCTTCAACCGGGTTATTCT	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.3	57.9	1656 bp	1.0	76.8
rpl2_p4	CGCTTCAACCGGGTTATTCT	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.3	57.9	1658 bp	1.0	76.8
rpl2_p5	ATCATTACGCTTCAACCGGG	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.3	58.1	1678 bp	1.0	76.3