Hotspot

trnH-GUG-rps19

IRa · 157402-157537 · 136 bp

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Pi 0.0221

MarkerSeek score 65.20

Primer available yes

Label trnH-GUG-rps19

Feature Metadata

label_name: trnH-GUG-rps19
feature_type: igs
region: IRa
length_bp: 136
variable_sites: 3
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rps19_p1	GATCCACTTGGCTACATCCG	TGCCCGAGACCAAGTTACTA	55.0	50.0	58.1	58.1	268 bp	1.0	79.3
trnH-GUG-rps19_p2	CAATCCACTGCCTTGATCCA	TGCCCGAGACCAAGTTACTA	50.0	50.0	57.9	58.1	282 bp	1.0	79.2
trnH-GUG-rps19_p3	AATCCACTGCCTTGATCCAC	TGCCCGAGACCAAGTTACTA	50.0	50.0	57.9	58.1	281 bp	1.0	79.2
trnH-GUG-rps19_p4	ACAATCCACTGCCTTGATCC	TGCCCGAGACCAAGTTACTA	50.0	50.0	57.9	58.1	283 bp	1.0	79.2
trnH-GUG-rps19_p5	TAATGTGATGGGCGAACGAC	TGCCCGAGACCAAGTTACTA	50.0	50.0	58.4	58.1	331 bp	1.0	77.3