Hotspot

trnV-GAC-rps12

IRa · 140776-142743 · 1968 bp

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Pi 0
MarkerSeek score 59.00
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1968
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 CGGGGGAGCAAGTCAAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 2101-2125 bp 1.0 77.9
trnV-GAC-rps12_p2 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 2040-2064 bp 1.0 77.6
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 2044-2068 bp 1.0 77.5
trnV-GAC-rps12_p4 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 2039-2063 bp 1.0 77.5
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 2042-2066 bp 1.0 76.6