Hotspot

rps15-ycf1

SSC · 125891-126278 · 388 bp

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Pi 0.00773

MarkerSeek score 58.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 388
variable_sites: 3
pi: 0.00773
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGA	38.1	45.0	55.5	56.3	576 bp	1.0	40.4
rps15-ycf1_p2	TCAACAGAACCCTTGTTTTCTTC	TGGCCCAATTACCGATTAGA	39.1	45.0	57.4	56.3	576 bp	1.0	40.4
rps15-ycf1_p3	CAACAGAACCCTTGTTTTCTTCT	TGGCCCAATTACCGATTAGA	39.1	45.0	57.4	56.3	575 bp	1.0	40.4
rps15-ycf1_p4	TGAAATTCAACAGAACCCTTGT	TGGCCCAATTACCGATTAGA	36.4	45.0	56.4	56.3	582 bp	1.0	40.4
rps15-ycf1_p5	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGAA	38.1	42.9	55.5	57.1	576 bp	1.0	40.4