Hotspot

rps19-trnH-GUG

IRb · 86040-86175 · 136 bp

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Pi 0.0221

MarkerSeek score 65.20

Primer available yes

Label rps19-trnH-GUG

Feature Metadata

label_name: rps19-trnH-GUG
feature_type: igs
region: IRb
length_bp: 136
variable_sites: 3
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-trnH-GUG_p1	TGCCCGAGACCAAGTTACTA	GATCCACTTGGCTACATCCG	50.0	55.0	58.1	58.1	268 bp	1.0	79.3
rps19-trnH-GUG_p2	TGCCCGAGACCAAGTTACTA	CAATCCACTGCCTTGATCCA	50.0	50.0	58.1	57.9	282 bp	1.0	79.2
rps19-trnH-GUG_p3	TGCCCGAGACCAAGTTACTA	AATCCACTGCCTTGATCCAC	50.0	50.0	58.1	57.9	281 bp	1.0	79.2
rps19-trnH-GUG_p4	TGCCCGAGACCAAGTTACTA	ACAATCCACTGCCTTGATCC	50.0	50.0	58.1	57.9	283 bp	1.0	79.2
rps19-trnH-GUG_p5	TGCCCGAGACCAAGTTACTA	TAATGTGATGGGCGAACGAC	50.0	50.0	58.1	58.4	331 bp	1.0	77.3