Hotspot

trnK-UUU-rps16

LSC · 4109-4848 · 740 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
740
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCTGGACGAATCTGTTGC GCTCAACCTACGGGAACTG 50.0 57.9 57.9 57.9 911 bp 1.0 70.2
trnK-UUU-rps16_p2 GTCTGGACGAATCTGTTGCT GCTCAACCTACGGGAACTG 50.0 57.9 57.9 57.9 910 bp 1.0 70.2
trnK-UUU-rps16_p3 AGTCTGGACGAATCTGTTGC GTGCTCAACCTACGGGAAC 50.0 57.9 57.9 58.2 913 bp 1.0 70.0
trnK-UUU-rps16_p4 GTCTGGACGAATCTGTTGCT GTGCTCAACCTACGGGAAC 50.0 57.9 57.9 58.2 912 bp 1.0 70.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 854 bp 1.0 69.5