Hotspot

psbA

LSC · 44809-45870 · 1062 bp

Back to Wallisia

Pi 0

MarkerSeek score 30.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACCCAATATCTTGCTAAACA	GTGCTTGGGAGTCCTTGAAA	35.0	50.0	52.1	58.0	1172 bp	1.0	40.0
psbA_p2	TGCTAAACAAGATATTGGGT	GTGCTTGGGAGTCCTTGAAA	35.0	50.0	52.1	58.0	1161 bp	1.0	40.0
psbA_p3	ACCCAATATCTTGCTAAACAA	GTGCTTGGGAGTCCTTGAAA	33.3	50.0	53.1	58.0	1172 bp	1.0	40.0
psbA_p4	TTGCTAAACAAGATATTGGGT	GTGCTTGGGAGTCCTTGAAA	33.3	50.0	53.1	58.0	1162 bp	1.0	40.0
psbA_p5	TGCTAAACAAGATATTGGGT	AATACGTGTGCTTGGGAGTC	35.0	50.0	52.1	57.9	1168 bp	1.0	40.0