Hotspot

rps19-psbA

LSC · 44664-44808 · 145 bp

Back to Wallisia

Pi 0.0138

MarkerSeek score 60.40

Primer available yes

Label rps19-psbA

Feature Metadata

label_name: rps19-psbA
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 2
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-psbA_p1	TCTGACTTTCGCGAGACATG	CCCCTAGACCTAGCTGTTGT	50.0	55.0	58.0	58.1	239 bp	1.0	79.5
rps19-psbA_p2	TCTGACTTTCGCGAGACATG	AACTTCCCCCTAGACCTAGC	50.0	55.0	58.0	57.8	245 bp	1.0	79.1
rps19-psbA_p3	TCTGACTTTCGCGAGACATG	AGCTGTTGTTGAAGCTCCAT	50.0	45.0	58.0	57.7	228 bp	1.0	78.3
rps19-psbA_p4	TCTGACTTTCGCGAGACATG	CTTCCCCCTAGACCTAGCTG	50.0	60.0	58.0	58.3	243 bp	1.0	78.2
rps19-psbA_p5	CCTACTCTGACTTTCGCGAG	CCCCTAGACCTAGCTGTTGT	55.0	55.0	57.8	58.1	244 bp	1.0	78.1