Hotspot

rpl32-trnL-UAG

SSC · 2973-3732 · 760 bp

Back to Wallisia

Pi 0.00132
MarkerSeek score 59.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
760
variable_sites
1
pi
0.00132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CACCGGGCATTCAAAAAGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.5 58.0 847 bp 1.0 75.8
rpl32-trnL-UAG_p2 CACCGGGCATTCAAAAAGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.5 58.0 848 bp 1.0 75.8
rpl32-trnL-UAG_p3 CACCGGGCATTCAAAAAGTT TTTAGAAGATGCCGCCACTC 45.0 50.0 57.5 58.0 907 bp 1.0 75.7
rpl32-trnL-UAG_p4 TTCCACCGGGCATTCAAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.6 58.0 850 bp 1.0 75.3
rpl32-trnL-UAG_p5 TTTCCACCGGGCATTCAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.6 58.0 851 bp 1.0 75.3