Hotspot

rpl16

LSC · 84358-86044 · 1687 bp

Back to Wallichia

Pi 0.00838
MarkerSeek score 64.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1687
variable_sites
14
pi
0.00838
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AAAAATAAACCTGCGGTTGT AGGTTGAATAAAAATTCGATTGACCA 35.0 30.8 54.3 58.1 1788-1847 bp 1.0 41.3
rpl16_p2 AAAATAAACCTGCGGTTGTT AGGTTGAATAAAAATTCGATTGACCA 35.0 30.8 54.3 58.1 1787-1846 bp 1.0 41.3
rpl16_p3 AAATAAACCTGCGGTTGTTT AGGTTGAATAAAAATTCGATTGACCA 35.0 30.8 54.3 58.1 1786-1845 bp 1.0 41.3
rpl16_p4 AATAAACCTGCGGTTGTTTT AGGTTGAATAAAAATTCGATTGACCA 35.0 30.8 54.3 58.1 1785-1844 bp 1.0 41.3
rpl16_p5 ATAAACCTGCGGTTGTTTTT AGGTTGAATAAAAATTCGATTGACCA 35.0 30.8 54.3 58.1 1784-1843 bp 1.0 41.3