Hotspot

ndhF-rpl32

SSC · 116761-117253 · 493 bp

Back to Wallichia

Pi 0.0153
MarkerSeek score 69.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
493
variable_sites
7
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT ACCGATTTCGCTAAAGAAAAAGA 40.0 34.8 55.3 57.1 706-714 bp 1.0 44.6
ndhF-rpl32_p2 TTGTTGCTGTCGGAATAAGT GAAACCGATTTCGCTAAAGA 40.0 40.0 55.3 54.1 709-717 bp 1.0 44.6
ndhF-rpl32_p3 TTGTTGCTGTCGGAATAAGT GAAACCGATTTCGCTAAAGAA 40.0 38.1 55.3 54.9 709-717 bp 1.0 44.6
ndhF-rpl32_p4 TTTGTTGCTGTCGGAATAAGT ACCGATTTCGCTAAAGAAAAAGA 38.1 34.8 56.1 57.1 707-715 bp 1.0 44.6
ndhF-rpl32_p5 TTTGTTGCTGTCGGAATAAGT GAAACCGATTTCGCTAAAGA 38.1 40.0 56.1 54.1 710-718 bp 1.0 44.6