Hotspot

psaJ-rpl33

LSC · 69903-70486 · 584 bp

Back to Wallichia

Pi 0.00358
MarkerSeek score 64.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
584
variable_sites
2
pi
0.00358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TGGTTTGGGTCTTTAGCAGG TGACACCATTTCGGACACAA 50.0 45.0 57.7 57.7 712-738 bp 1.0 78.1
psaJ-rpl33_p2 TGGTTTGGGTCTTTAGCAGG TTGACACCATTTCGGACACA 50.0 45.0 57.7 57.7 713-739 bp 1.0 78.1
psaJ-rpl33_p3 GGTTTGGGTCTTTAGCAGGT TGACACCATTTCGGACACAA 50.0 45.0 57.7 57.7 711-737 bp 1.0 78.1
psaJ-rpl33_p4 GGTTTGGGTCTTTAGCAGGT TTGACACCATTTCGGACACA 50.0 45.0 57.7 57.7 712-738 bp 1.0 78.1
psaJ-rpl33_p5 TGGTTTGGGTCTTTAGCAGG ACACCATTTCGGACACAACT 50.0 45.0 57.7 57.7 710-736 bp 1.0 78.1