Hotspot

accD-psaI

LSC · 61454-62356 · 903 bp

Back to Wallichia

Pi 0.0023

MarkerSeek score 63.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 2
pi: 0.0023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	989-1017 bp	1.0	81.0
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	988-1016 bp	1.0	81.0
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	995-1023 bp	1.0	80.3
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	994-1022 bp	1.0	80.3
accD-psaI_p5	TTGACCCAATTGTACCACGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	1043-1071 bp	1.0	77.4