Hotspot

trnT-UGU-trnL-UAA

LSC · 47258-48380 · 1123 bp

Back to Wallichia

Pi 0.00739

MarkerSeek score 59.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 8
pi: 0.00739
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	1173-1190 bp	1.0	82.2
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	1194-1211 bp	1.0	82.1
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1181-1198 bp	1.0	81.6
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1205-1222 bp	1.0	81.2
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	ACAATCAAGTCCGTAGCGTC	50.0	50.0	57.8	58.0	1226-1243 bp	1.0	81.1