Hotspot

psbC-trnS-UGA

LSC · 36085-36225 · 141 bp

Back to Wallichia

Pi 0.0567

MarkerSeek score 82.10

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 8
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	248 bp	1.0	80.8
psbC-trnS-UGA_p2	CTGTTCTTTCCATGACCCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	247 bp	1.0	80.8
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	287 bp	1.0	80.7
psbC-trnS-UGA_p4	CTGTTCTTTCCATGACCCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	258 bp	1.0	80.2
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	298 bp	1.0	80.1