Hotspot

atpH-atpI

LSC · 14545-15227 · 683 bp

Back to Wallichia

Pi 0

MarkerSeek score 62.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	742-744 bp	1.0	77.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	770-772 bp	1.0	77.4
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	825-827 bp	1.0	77.2
atpH-atpI_p4	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	58.0	58.6	853-855 bp	1.0	77.1
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	826-828 bp	1.0	76.6