Hotspot

ycf1

SSC · 122499-128162 · 5664 bp

Back to Vuhuangia

Pi 0.000883

MarkerSeek score 51.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5664
variable_sites: 5
pi: 0.000883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAAACGACTGCCATTATTGG	AACGTCGTAGAAAAGTGAGA	42.9	40.0	56.9	54.4	252 bp	1.0	40.3
ycf1_p2	TGAAACGACTGCCATTATTGG	ACGTCGTAGAAAAGTGAGAA	42.9	40.0	56.9	54.4	251 bp	1.0	40.3
ycf1_p3	GAAACGACTGCCATTATTGGT	AACGTCGTAGAAAAGTGAGA	42.9	40.0	56.9	54.4	251 bp	1.0	40.3
ycf1_p4	GAAACGACTGCCATTATTGGT	ACGTCGTAGAAAAGTGAGAA	42.9	40.0	56.9	54.4	250 bp	1.0	40.3
ycf1_p5	TGAAACGACTGCCATTATTGG	AACGTCGTAGAAAAGTGAGAA	42.9	38.1	56.9	55.3	252 bp	1.0	40.3