Hotspot

ndhF-rpl32

SSC · 111631-112044 · 414 bp

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Pi 0.00483

MarkerSeek score 57.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 2
pi: 0.00483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	AAGAAAACGCCTTTAACGCC	50.0	45.0	57.7	57.6	566 bp	1.0	74.9
ndhF-rpl32_p2	CTGGAAGTGGAACGAAAGGT	CGCCCAATAGCCTTTCCTTT	50.0	50.0	57.7	58.5	547 bp	1.0	74.0
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	CGCCCAATAGCCTTTCCTT	50.0	52.6	57.7	57.8	547 bp	1.0	68.7
ndhF-rpl32_p4	CTGGAAGTGGAACGAAAGGT	CCTTTAACGCCGCCCAATA	50.0	52.6	57.7	58.2	557 bp	1.0	68.5
ndhF-rpl32_p5	CTGGAAGTGGAACGAAAGGT	AAAGAAAACGCCTTTAACGCC	50.0	42.9	57.7	58.3	567 bp	1.0	68.1